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CSMC - Flow Cytometry Core: Pre-Sort Form

Required fields are marked with an *

  • COMPLETION MANDATORY TO HOLD SORT APPOINTMENT
  • Single color compensation tubes REQUIRED
  • Basic cell preparation protocol is available at Flow Core
  • Cells need to be filtered and be at 30-40 million/mL concentration to be sorted at optimal speed
  Name Phone #
PI *
User *
SORT DATE RESERVED* (mm/dd/yyyy)  Account #*
Instrument Selection
If the sample is human and/or biohazardous it must be run on the Aria III
Is your sample biohazardous? (NO, if only potential hazard is human sample)*If YES, what?
  • An additional 30 min. is included to account for setup and cleanup
  • Price is just an approximation; users will be billed in 15 minute increments for actual time of the sort
  • Fill in the "Total # of cells". in millions, you will be sorting in the table below for your preferred instrument and nozzle
  • After Hours sorting (after 5pm) will be charged an extra $15/hour
2A. ARIA IINO HUMAN OR BIOHAZARDOUS SAMPLES($80/hr)
Nozzle Size
(µm)
Sort Rate
Estimation(Events/hr)
Total # of Cells to SORT
(in Millions)
FILL-IN-HERE
Hours Needed
(Includes extra 30 min)
Total Price Estimate
70(8-10 µm cells) 60 million
 
 
80(10-12 µm cells) 33 million
 
 
100(12-14 µm cells) 22 million
 
 
2B. Aria III HUMAN OR BIOHAZARDOUS SAMPLES ALLOWED($90/hr)
Nozzle Size
(µm)
Sort Rate
Estimation(Events/hr)
Total # of Cells to SORT
(in Millions)
FILL-IN-HERE
Hours Needed
(Includes extra 30 min)
Total Price Estimate
(Calculated for Aria, except 140 nozzle)
70(8-10 µm cells) 60 million
 
 
85(10-12 µm cells) 33 million
 
 
100(12-14 µm cells) 22 million
 
 
130(14-22 µm cells) 5 million
 
 
(Influx Only)
140(14-25 µm cells)
18 million
 
 
Sort Setup
Cell Type Species
Fluorochromes used
  Fluorochrome Molecule
1
2
3
4
5
6
7
8
9
10
Cell Populations to be sorted
    % Expected
1
2
3
4
How do you want to collect your cells?* What will the sorted cells be used for?
Version:1.1.1